ESSENZIALE DI BIOLOGIA MOLECOLARE DELLA CELLULA ALBERTS PDF

The course is designed to provide a basic knowledge of biochemical, biological and genetic principles necessary for graduate study in midwifery. At the end of the course the student must know the chemical composition of living organisms and their constituents, the structures and functions of cells and the related biochemical processes in biological and pathophysiological conditions. The student must know the main metabolic processes at the molecular level, their integration into the body, the genome organization and the main mechanisms underlying the expression and transmission of the genetic information. The student must also know the main biophysical and biochemical methods with particular attention to topics of diagnostic pre-natal and post-natal relevance. The course also aims: - to provide basic knowledge on the normal functioning of the body in order to support the understanding of the pathological changes and and provide principles for the development of decision-making tools.

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Servizi per la didattica. Teacher Status SSD h. Les h. Lab h. Tut Years teaching. Espandi Riduci Nessuna collaborazione prevista.

Date d'appello. Orario delle lezioni. Statistiche superamento esami. Presentazione Course description. La seconda parte, relativa alla biologia molecolare e microbiologia ha i seguenti obiettivi formativi: - la conoscenza di base della biologia cellulare e molecolare morfologia e fisiologia cellulare, meccanismi molecolari alla base della trasmissione dell'informazione e dell'espressione genica e della biochimica cellulare.

The second part is molecular biology and microbiology and the main topics are: - basics of cellular and molecular biology cellular morphology and physiology, molecular mechanisms of the transmission of information, gene expression and cell biochemistry. Risultati attesi Expected Learning Outcomes.

The student will have to acquire the fundamentals of cellular systems application in industrial biotechnology field. At the end of the course, the student will be asked for: - knowledge of classes of biological macromolecules - knowledge of cellular structure and microorganisms - knowledge of cellular metabolism fundamentals - knowledge of food microbiology fundamentals. Prerequisiti Prerequirements. L'allievo deve avere acquisito i fondamenti della chimica organica classi di composti organici e meccanismi principali delle reazioni chimiche.

Students should know the basic concepts of general chemistry and organic chemistry. Programma Course topics. Composizione chimica della cellula Le principali molecole organiche di interesse biologico amminoacidi, nucleotidi, glucidi, acidi grassi : struttura e funzione. Le macromolecole biologiche polisaccaridi, lipidi, acidi nucleici e proteine.

Struttura, funzione e metabolismo del DNA in procarioti ed eucarioti: meccanismi molecolari e regolazione dei processi di replicazione. Struttura, funzione e metabolismo degli RNA in procarioti ed eucarioti: meccanismi molecolari e regolazione del processo di trascrizione. Sintesi proteica in procarioti ed eucarioti: attivazione degli amminoacidi, codice genetico ed interazione codone-anticodone, struttura dei ribosomi, fasi della sintesi proteica.

Struttura e funzione delle proteine: proteine fibrose, globulari, enzimi. Sintesi chimica di peptidi. Cenni di ingegneria proteica: la mutagenesi mirata e la manipolazione delle proteine. La tecnologia del DNA ricombinante Isolamento e caratterizzazione dei geni: enzimi di restrizione, vettori di clonaggio, preparazione e screening di banche genomiche. La manipolazione dell'espressione genica nei procarioti.

La produzione di proteine ricombinanti negli eucarioti. Colture cellulari e animali transgenici Metabolismo cellulare Vie cataboliche e vie anaboliche. Energia libera delle reazioni biochimiche. Idrolisi dei composti fosforilati. Legami energetici. Metabolismo dei carboidrati: la glicolisi ed i suoi meccanismi di regolazione.

La gluconeogenesi. Il ciclo degli acidi carbossilici: regolazione e significato del ciclo, vie anfiboliche, reazioni anaplerotiche. Trasporto degli elettroni e fosforilazione ossidativa. Classi di microrganismi di interesse biotecnologico: batteri, lieviti, funghi filamentosi.

Microbiologia industriale: principali processi e prodotti biotecnologici biomasse microbiche, acidi organici, antibiotici Microbiologia alimentare: microrganismi e trasformazione degli alimenti. Le tossinfezioni alimentari. Controllo della crescita microbica negli alimenti. Characteristics of living organisms: cellular structure, metabolism, gene expression, reproduction and evolution. Chemical composition of cell.

Biological organic molecules amminoacids, nucleotides, carbohydrates, fatty acids. Biomacromolecules polysaccharides, lipids, nucleic acids and protein. Basics of vitamins. Protein synthesis: amino-acid activation, genetic code, codon-anticodon interaction, ribosome structure.

Protein structure and function: fibrous and globular proteins, enzymes. Chemical synthesis of peptides. Basics of protein engineering.

Recombinant DNA technology. Isolation and characterization of genes: restriction enzymes, cloning vectors, microbial and cellular expression systems, pharming.

Cellular metabolism. Catabolic and anabolic pathways: control of metabolic flux. ATP and "high-energy" compounds. Coupled reactions. Redox reaction. Metabolism of carbohydrates: glycolysis and gluconeogenesis. Krebs cycle: mechanisms and regulation, electron transport and oxidative phosphorilation.

Cellular systems in biotechnology and food microbiology. General features of prokaryotic and eukaryotic cells: cellular structure and organization, reproduction. Microorganism classes: bacteria, yeasts, and filamentous fungi.

Industrial microbiology: biotechnological products microbial biomasses, organic acids, antibiotics. Food microbiology: microorganisms and food interactions, food pathogens, control of microorganism growth in food.

Note Additional information. Organizzazione dell'insegnamento Course structure. In the second part of the course molecular biology and microbiology , lab work includes the extraction of nucleic acids from different vegetal sources.

The concentration and purity of extracted nucleic acids and proteins in the sample will be determined by means of UV-Vis spectroscopy. Bibliografia Reading materials. I testi di riferimento consigliati sono: I testi di riferimento consigliati per biologia molecolare e microbiologia sono: B. Alberts, A. Johnson, J.

Lewis, M. Raff, K. Roberts, P. Nelson, M. Farris, M. Gobbetti, E. Neviani; M. Microbiologia dei prodotti alimentari I testi per approfondimenti sono: T. Handouts will be available on the web page of the course.

The recommended textbooks for molecular biology and microbiology: B. Microbiologia dei prodotti alimentari Advanced textbook: T. Regole d'esame Assessment and grading criteria. Exam: compulsory oral exam; practical lab skills test;. For the second part of the course molecular biology and microbiology : oral test maximum 28 marks. Report of results obtained in lab tests will be evaluated maximum 2 marks.

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L'essenziale di biologia molecolare della cellula

Servizi per la didattica. Teacher Status SSD h. Les h. Lab h. Tut Years teaching. Espandi Riduci Nessuna collaborazione prevista. Date d'appello.

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Bruce Alberts

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