MANUAL DE KINESIOLOGIA ESTRUCTURAL PDF

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The intranuclear occurrence and fine structural details of schizonts of Perezia fumifeanae Microsporida: Nosematidae. Lepidoptera: Tortri- cidae. Perezia fumiferanae Microsporida: Nosernatidae , a parasite of Choristoneura fumiferana Lepidop- tera: Tortricidae , occurs within several tissues of the host.

Schizonts occur within both nucleus and cytoplasm and are separated from the host only by their own membrane. The ultrastructure of the schizonts supports the suggestion that P. Perezia fumiferanae was described as a para- site of Choristoneura fumiferana by Thompson 6.

It has recently been considered for reclassi- fication with the suggestion that it is a species of the type genus, Nosema 8. Ultrastructural investigations of the development of N. The schizonts are truly intracytoplasmic, i. Tubular extensions of the membrane project into the host cytoplasm. The cytoplasm of the schizont contains ribosomes, smooth endoplasmic reticulum, rough endo- plasmic reticulum, and Golgi complexes, but no mitochondria. The nucleus is a diplokaryon with little chromatin and no nucleolus.

Host cell mitochondria, rough endoplasmic reticulum, and Golgi complexes are sometimes in close association with the schizonts. This report describes some additional aspects of the ultra- structure of N. Female C. Stocks were supplied from Sault Ste. Marie which had been reared in the laboratory on a synthetic diet 2.

Tissue was fixed with cold 2. Silver to grey sections were observed and photographed with- out further staining. In addition, sections from tissue fixed only in glutaraldehyde were reacted with silver methenamine to show complex carbohydrates following the method of Ram- bourg 4. Light gold sections were oxidized in ITo periodic acid for 10 min and reacted with the silver methenamine solution in the dark at 60C for 10 min.

This step blocked existing aldehydes, either naturally occurring or those which had been introduced by the fixative. All specimens were placed on uncoated. IContribution No. Adult C. Box , Sault Ste. Marie, Ontario. Stages in the development of N.

Schizonts are easily overlooked because there is usually very little contrast between their cytoplasm and that of the host cell Fig. The schizonts, usually intracytoplasmic, have the known characteristics of the three Nosema species described above , most of which are illustrated in Figs. Tubular extensions at the limiting membrane are occasionally observed Fig. Schizonts were seen within the nucleus of two cells Fig. Each schizont is surrounded only by its own limited membrane Fig.

The structure of the host nucleus seemed normal. The limiting membrane does not reduce silver in sections reacted with silver methenamine solution with or without periodic acid oxidation Figs. In contrast to the limiting membrane the external layer of numerous everted polar filaments reacts strongly with silver methenamine solution, with or without oxidation Fig.

We may therefore conclude that the limiting membrane does not contain periodic acid reactive complex carbo- hvdrates. The close resemblance of the structure of the schizonts of N. The membrane of N. Schizonts of N. Sincere thanks are also extended to Dr. Locke for constructive criticism and to Dr. Weatherston for advice during preparation of the report. CALI, A. Morphogenesis in the genus Noserna. IV Int. Insect Pathol. An improved laboratory method for rearing large numbers of spruce budworm, Choris- toneura furnifeana Lepidoptera: Tortricidae.

A routine method for obtaining high contrast without staining sections. Cell Biol. An improved silver methena- mine technique for the detection of periodic acid - reactive complex carbohydrates with the electron microscope. The ultra- structure of Encephalitozoon cuniculi Microsporida: Nosematidae and its taxonomic simcance. Perezia furnifeanae n. Nosema algerae n. Cnidospora, Microsporida a pathogen in a laboratory colony of Anopheles stephensi Liston Dip- tera, Culcidae.

Studies on Nosema fumiferanae a microsporidian parasite of Choristoneura furniferana Clem. Lepidoptera: Tortricidae. Come11 University, Ithaca, N. The fhe structure of the developmental stages of the micro- sporidian Noserna apis Zander. Tissue Cell, 3: Lotated in fat body cell. X 25 X 22 Golgi complex of schizont. X 79 8OO. Tubular extensions of lirnitrng membrane. X 79 Intranuclear schizont in modified epidermal cell of sex pheromone gland. Open arrow indicates area enlarged in insert.

X Unoxidized control. Appearance of schizonts which had been blocked with sodium metabisulfite and oxidized before reacting in silver methenamine solution. Log in Get Started. The intranuclear occurrence and fine structural details of schizonts of Download for free Report this document. Embed Size px x x x x Transcript of The intranuclear occurrence and fine structural details of schizonts of All specimens were placed on uncoated IContribution No.

Zoo l. D ownl oade d fr om w ww. Acknowledgments The author is grateful to Dr. Wilson for the identification of the parasite. Z ool. Dow nloa ded from ww w.

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The intranuclear occurrence and fine structural details of schizonts of Perezia fumifeanae Microsporida: Nosematidae. Lepidoptera: Tortri- cidae. Perezia fumiferanae Microsporida: Nosernatidae , a parasite of Choristoneura fumiferana Lepidop- tera: Tortricidae , occurs within several tissues of the host. Schizonts occur within both nucleus and cytoplasm and are separated from the host only by their own membrane.

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Manual de Kinesiologia Estructural

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